The effect of a high fat diet on glycogen , blood composition , and endurance performance after treadmill exercise in rats

Journal of Exercise Nutrition & Biochemistry 2000;4(2):49-62.

Abstract

I
t is well known that high fat diet enhances using of fatty acids during the last stage of endurance exercise. But effects of high fat diet administration on endurance exercise were not well documented. At this point of view, we ingested intralipose and a high fat diet in rats to increase fatty acids utilization during endurance exercise performance. Thirty male Sprague-Dauley rats were chosen as the subjects in this experiment. These subjects were divided into two groups on the basis of diet protocols as follows : ① normal diet(carbohydrates 58%, proteins 23.8%, lipids 8%, and others), ② high fat diet(carbohydrates 45%, proteins 25%, lipids 30%). And in high fat diet group, intralipose was ingested before exercise. Endurance exercises on the basis of Bruce protocol were applied to the groups. Serum glucose, lactic acid, FFA, TG, cholesterol concentration were analyzed. Liver, soleus and gastrocnemius white muscle glycogen contents were determined and endurance exercise time was measured. As a result of experiment, high fat diet group was decreased less than normal diet group in serum glucose concentration and in liver, soleus and gastronemius white muscle glycogen contents. On the other hand, serum FFA and TG concentration were increased more high fat diet group than nomal diet group, and serum lactic acid concentration were increased less high fat diet group than normal diet group. And endurance exercise time was increased after a single high fat diet. In conclusion, even though a single high fat diet, glycogen-sparing effect in rats was occurred.