Articles : Changes of Blood Metabolites, Hormones and Cytokines Levels after Treadmill Running Exercise and Energy Restriction for 6 Weeks in Obese Rats |
|
Abstract |
This study examined effects of exercise and energy restriction on changes of blood levels of hormones and cytokines in obese rats. Subjects were consisted of 2 weeks-aged male Sprague-Dawley rats (N = 96), and were divided into 6 groups as control, obese, and 4 intervention groups (Diet alone, Exercise alone, Diet plus exercise type A, and Diet plus exercise type B). Serum concentrations of glucose, FFA, insulin, growth hormone, cortisol, and leptin, plasma TNF-α, and IL-6 levels were measured at pre and post-treatment of 6 weeks. After 6 weeks of treatment, all groups resulted in a significant (F = 18.624, p<.001) reduction of serum glucose levels, compared with pre-treatment. Serum FFA levels were significantly (F = 53.823, p<.001) decreased in all groups after treatment, but the result of post hoc demonstrated the significant differences after treatment in exercise group (F = 37.533, p<.001) and combine B group (F = 17.316, p<.05), respectively. In compared with pre-treatment measurements, serum insulin level was significantly decreased after 6 weeks of treatment in exercise group (F = 77.725, p<.01) and combine B group (F = 165.787, p<.001), respectively. Serum cortisol level was significantly increased in obese group (F = 28.840, p<.05) and exercise group (F = 8.110, p<.05) after 6 weeks of treatment as compared with pre-treatment measurements, although the other groups showed a decreasing tendency between pre-treatment and post-treatment. Plasma IL-6 level was significantly (F = 25.838, p<.001) increased in all groups after 6 weeks of treatment as compared with pre-treatment level, but there were no differences among groups at post-treatment. In summary, responses of serum insulin and FFA levels in weight loss intervention implied that insulin sensitivity for lipolytic activation was improved by a sufficient amount of exercise. Plasma IL-6 and TNF-α levels in all intervention groups showed a similar responses after intervention of 6 weeks, so it could not confirm a significant difference of cytokines related to immune function among different intervention. Further research is needed in the analysis of muscle and liver tissues before meaningful mechanism can be drawn. |
Key Words:
obesity, diet plus exercise, insulin, IL-6, TNF-α |
|